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SRX2188783: RNA-Seq of mixotrophic of mixotrophic protists Micromonas and Pyramimonas
1 ILLUMINA (Illumina MiSeq) run: 51.3M spots, 10.4G bases, 3.7Gb downloads

Design: Pyramimonas culture was grown under nutrient limiting conditions which favor mixotrophy Cells were harvested by filtration onto 0.4 micron pore size polycarbonate membrane and lysed using the Qiagen RNeasy Mini Kit. DNA was removed by DNase treatment on the purification column. Total RNA quality was checked by Bioanalyzer and sent to Sulzberger Columbia Genome Center for library preparation and RNA-Seq. mRNA was collected by poly-A pulldown, then reverse transcribed into cDNA. The material was fragmented and then adapter/primers added and finally sequenced.
Submitted by: Woods Hole Oceanographic Institution
Study: Micromonas sp. (CCMP 2099) and Pyramimonas tychotreta Transcriptomes
PRJNA342459 • SRP090401 • All experiments • All runs
show Abstracthide Abstract
Comparison of gene expression from two genera of polar mixotrophic microalgae under conditions that stimulate photosynthetic activity or phagotrophic activity
Sample: 50ml culture of alga under reduced nutrient conditions (f/20 medium) to stimulate mixotrophic activity
SAMN05757691 • SRS1711533 • All experiments • All runs
Organism: Pyramimonas tychotreta
Library:
Name: Pyramimonas 8
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs: 1 run, 51.3M spots, 10.4G bases, 3.7Gb
Run# of Spots# of BasesSizePublished
SRR429331551,296,21810.4G3.7Gb2017-10-02

ID:
3184009

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